Sequences of crr, cya, fruR, and ptsHI unchanged at generation 2000
Note: The following material is unpublished. Please cite Michael Travisano (personal communication) and this web site if you use these sequence data.
Mike Travisano sequenced 1323 base-pairs (bp) in a clonal sample from each of the 12 long-term populations at generation 2000. The regions sequenced included the upstream promoters and adjacent coding regions for crr, cya, fruR, and ptsHI. These particular genes were chosen based on evidence that regulatory changes in the phosphotransferase system may have contributed to the genetic adaptation to the glucose-limited environment (Travisano and Lenski 1996). However, there were no mutations whatsoever from the ancestral sequence among 15876 (=12 x 1323) bp sequenced.
The following shows the DNA sequences obtained for the E. coli B founding strain. Differences between this strain and E. coli K12 are shown in bold red. There are very few differences -- only 3 out of 1323 bp -- which is lower than most sequence comparisons between B and K12 (Milkman 1996*) and suggests that these regions are highly conserved.
* Milkman, R. 1996. Recombinational exchange among clonal populations, pp. 2663-2684 in Escherichia coli and Salmonella: Cellular and Molecular Biology, edited by F. C. Neidhardt. ASM Press, Washington, DC.
Gene region: crr. 339 bp sequenced.
No differences between B and K12.
CGTGGTAATGATATGATTTCACACCTTTACCAGCCAATGTCACCGTCCGTGCTGAACTTG
ATCAAGCAAGTTATTGATGCTTCTCATGCTGAAGGCAAATGGACTGGCATGTGTGGTGAG
CTTGCTGGCGATGAACGTGCTACACTTCTGTTGCTGGGGATGGGTCTGGACGAATTCTCT
ATGAGCGCCATTTCTATCCCGCGCATTAAGAAGATTATCCGTAACACGAACTTCGAAGAT
GCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTG
GTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCC
Gene region: cya. 284 bp sequenced.
Two differences between B and K12; both
C are
T in K12.
GGCAATTCTTAAAACATTGTCTAACATGCTTGTTACCGTCATTATCATCCGTGGTCCATC
CTAACATCCTTGCCAGAGCGATGTCAGTGTTGTGGTGAAACGTAGACGCCTGCGCAAACC
GTAAAATGAGGTCTGGCAGTGGATCCTGACAGGCGTTTCACGCCGTCGTAATAAGGAATT
TACAGAGAATAAACGGTGCTACACTTGTATGTAGCGCATCTTTCTTTACGGTCAATCAGC
AAGGTGTTAAATTGATCACGTTTTAGACCATTTTTTCGTCGTGA
Gene region: fruR. 360 bp sequenced.
One difference between B and K12; the T is
absent from K12.
AAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC
TTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGC
GCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTAC
GCAAGGGGCAATTGTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCAC
TGCAAGCTATGTTATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGA
AAAAGTCATGGCTGTGGTGCGTTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGC
Gene region: ptsHI. 340 bp sequenced.
No differences between B and K12.
GCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTT
CAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTA
ATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATG
ATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGT
CAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTG
GCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGA